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Archive for July, 2007

Biochemical Identification and Cytoenzymology

Thursday, July 5th, 2007

Biochemical Identification
There has been a number of demonstrable instances of cross-culture contamination. This occurs by manipulative errors, and though rare, the introduction of a single foreign cell, with slight growth rate advantage, can be sufficient to swamp the host population. The morphological similarity of mammalian cells in culture, as seen with biology microscopes, prevents […]

Serological Identifications and the Karyology of Cells in Culture

Thursday, July 5th, 2007

Serological Identifications of Cells in Culture
It is of great importance that cell species could be identified by serological as well as by other procedures with the use of biology microscopes. Fluorescein-labeled antibody is used for species identification of animal cells. Cells cultivated in vitro are readily identified with regard to their species of origin by […]

Prevention of Mycoplasma Contamination

Thursday, July 5th, 2007

Measures are used to control the sources and the vehicles of spread of mycoplasma contamination, which can be identified through biology microscopes. The major sources of contamination are of human, bovine, and swine origin. The major vehicles of spread are aerosols and contaminated reagents. The measures used are: the use of primary cell cultures whenever […]

The Control of pH with Synthetic Buffers

Thursday, July 5th, 2007

A series of hydrogen ion buffers covering the pKa range 6.15-8.60. Most of these buffers are amino acids. The synthetic buffer 4-2hydroxyethyl-1-piperazineethanesulfonic acid, HEPES, has gained widespread use recently.
The preservation of animal cells in liquid nitrogen, which may be seen under biology microscopes, is now a routine practice. Damage associated with freezing has been prevented […]

Osmolality in Culture Media

Thursday, July 5th, 2007

With the use of biology microscopes, it can be observed that living cells maintain an internal composition very different from, but highly dependent upon, their milieu. This is due to a combination of active and passive transport. In particular, cells respond by changes in size and shape to changes in the osmotic activity of […]

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